Cell Bio

Princy Mary Paulose
Bio 594
Research paper
05-25-2011













In-situ hybridization of tau mRNA in transgenic Drosophila melanogaster thirdly instar larvae














Abstract:

The purpose of this research was to admit the musical arrangement of human tau DNA in Drosophila melanogaster third instar larvae, and to detect the presence of tau mRNA wherever it was expressed. Drosophila fly transgenic for human tau were mated with flies having specific tissue-specific promoters which directed tau expression to specific tissues in the progeny. The promoter lines have specific GAL-4 transcription factors, which binds to UAS enhancer sequence upstream of tau promoter in the transgenic flies, bring forth the transcription of tau in specific tissues in the latter. The two GAL-4 patterns that were use in this research were elav and eyeless. The expression of tau mRNA, if any, has to be detected using in-situ hybridization using an anti-sense RNA investigating. The target-probe binding go forth be detected by antibodies, which specifically bind to regions where the probe mRNA (anti-sense) has bound to target mRNA (sense).



In the end, derived function interference contrast microscopy technique allow for be utilize for imaging of the desired gene expression. In this experiment, a detrimental control was also performed which involved a sense probe, which is not complement to the target mRNA (sense) of our interest. There will not be any target binding in this case.
We extend that the F1 progeny formed by crossing elav GAl4 lines and transgenic tau flies will express tau in the elav pattern. Similarly, the eyeless driver lines will dissolving agent in expression of tau in the same pattern in F1 generation.



Background and Significance:
1.Drosophila as the model existence:
The use of drosophila melanogaster, commonly known as payoff fly, as a model organism for genetic studies has remarkable success. It is said that studies in fruit flies...If you want to get a full essay, order it on our website: Orderessay



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